Figure 6. Uptake of glucose by M. smegmatis ML16.

Accumulation of [¹⁴C]glucose by M. smegmatis ML16 expressing wt MspA, empty vector pMS2, M1 MspA, and M1-M1₁₉ MspA was measured. The experiments were done in triplicates. The data are shown as averages ± standard deviations. The assay was performed at 37°C at a final glucose concentration of 1 µM. The cells were grown to an A₆₀₀ ∼0.6. At indicated time points 200 µL of cells were drawn from a vial, applied on a 0.22 µm cellulose filter, washed several times with LiCl, and counted on a scintillation counter. Dashed lines represent regression analysis of the first three data points for each strain. Uptake rates for ML16 expressing wt MspA, empty vector, M1 MspA, and M1-M1₁₉ MspA were 0.42, 0.01, 0.44, 0.24 nmol/mg cells/min, respectively.