Skip to main content
. 2012 Jun 18;7(6):e37860. doi: 10.1371/journal.pone.0037860

Figure 9. MCT8 and D3 immunoreactivities in axon varicosities of the rat parvocellular hypophysiotropic neurons.

Figure 9

Confocal images were subjected to deconvolution. Boxed areas are enlarged in B and C, respectively. The immunofluorescent signal for MCT8 (red) is distributed as small dots throughout the external zone of median eminence and appears (arrowheads) on the surface of gonadotropin-releasing hormone (GnRH, C), thyrotropin-releasing hormone (TRH, D), corticotropin-releasing hormone (E), growth hormone-releasing hormone (GHRH, F) and somatostatin (SST, E) immunofluorescent axon varicosities (green). (H,I) Representative images of triple immunofluorescent labelings demonstrate D3 (blue), MCT8 (red) and a hypophyseotroph hormone (green) in the axons of the median eminence. MCT8-immunoreactive puncta (arrowheads) appear on the surface of the following categories of axon varicosities; single-labeled for D3 (Ha), single-labeled for GnRH (Hb), and double labeled for D3 and GnRH (Hc) or CRH (I). Scale bars: A: 10 µm, B: 5 µm, C-I: 2 µm.