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. 2012 Jun;19(Suppl 1):S33–S44. doi: 10.3747/co.19.1149

FIGURE 1.

FIGURE 1

The dna extracted from the macrodissected tissue specimen was amplified using primers specific for exon 19 of the EGFR gene. The polymerase chain reaction product was then purified and sequenced in both directions using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA, U.S.A.). The sequences obtained were then compared with the U.S. National Center for Biotechnology Information reference sequence (EGFR: NM_005228.3). In this case, a deletion is observed to span nucleotides 2235–2249, which results in the deletion of amino acids at positions 746–750 (inclusive) in exon 19 of the EGFR gene.