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. Author manuscript; available in PMC: 2013 Jun 21.
Published in final edited form as: FEBS Lett. 2012 May 26;586(13):1813–1820. doi: 10.1016/j.febslet.2012.05.020

Figure 2. Characterization of FN up-regulated during TGFβ1-induced EMT in human lung carcinoma cells.

Figure 2

A549 cells (A and B) and NCI-H358 (C and D) were cultured and treated as described in M&M. Cell lysates (10 µg protein) were analyzed by SDS/PAGE and Western blot. Total FN, norFN and onfFN were detected with mAbs EP5, YKH1 and FDC6, respectively; Together with GAPDH used as loading control. Representative results from triplicate experiments are shown (A and C). Signal intensities were normalized, and relative intensities are shown as mean ± SD (B and D). ns: not significant; **P ≤0.005.