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. Author manuscript; available in PMC: 2013 Jun 21.

Published in final edited form as: FEBS Lett. 2012 May 26;586(13):1813–1820. doi: 10.1016/j.febslet.2012.05.020

Plates were coated with BSA, plasma FN (pFN), or total FN (tFN), and the cells were seeded on the coated plates as described in M&M. TGFβ1 treatment was used as a positive control. (I) For morphology changes, photos were taken by phase-contrast microscopy (Nikon) at 80X magnification. (II) Expression level of epithelial vs. mesenchymal cell markers were analyzed by Western blot using cell lysates (10 µg protein) of A549 (A, C, E and G) and NCI-H358 cells (B, D, F and H). Representative results from triplicate experiments are shown (A and B). Signal intensities were normalized, and relative intensities are shown as mean ± SD for Ecad (C and D), Ncad (E and F) and vimentin (G and H). ns: not significant; *P ≤ 0.05; **P ≤0.005.

Plates were coated with BSA, plasma FN (pFN), or total FN (tFN), and the cells were seeded on the coated plates as described in M&M. TGFβ1 treatment was used as a positive control. (I) For morphology changes, photos were taken by phase-contrast microscopy (Nikon) at 80X magnification. (II) Expression level of epithelial vs. mesenchymal cell markers were analyzed by Western blot using cell lysates (10 µg protein) of A549 (A, C, E and G) and NCI-H358 cells (B, D, F and H). Representative results from triplicate experiments are shown (A and B). Signal intensities were normalized, and relative intensities are shown as mean ± SD for Ecad (C and D), Ncad (E and F) and vimentin (G and H). ns: not significant; *P ≤ 0.05; **P ≤0.005.