Skip to main content
. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Free Radic Biol Med. 2012 May 1;53(1):1–11. doi: 10.1016/j.freeradbiomed.2012.04.021

Figure 3. Inhibition of hydrogen peroxide stimulation of Akt activity by 4-HNE in HepG2 cells.

Figure 3

Western blot and quantification of in vitro Akt kinase activity assay using a synthetic GSK3αβ peptide and increasing concentrations of 4-HNE (0, 10, 25, 50, 100μM) ± 1mM hydrogen peroxide. All samples performed in triplicate. Statistical analysis was via 1-way analysis of variance with Tukey’s multiple comparison test **p<0.01, ***p<0.001.