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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: Cancer Res. 2012 Apr 23;72(12):3069–3079. doi: 10.1158/0008-5472.CAN-11-4106

Figure 2. Mcl-1 levels are regulated by protein stabilization.

Figure 2

(A) Mcl-1 protein stability was determined following 10 μg/ml cycloheximide (CHX) treatment (LHS). (B) Nalm-6 and Nalm-6 ABT-R cells were pretreated with ABT-737 (500 nM) for 3 h prior to exposure to CHX to determine Mcl-1 protein stability (LHS). (C) Mcl-1 protein half-life was determined in Reh and Reh ABT-R cells following CHX treatment (LHS). Data in A–C were quantified by Image J software and the relative intensity of each lane was calculated with respect to the sample at 0 h. Results of a representative experiment (n=3) are shown.