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. Author manuscript; available in PMC: 2013 Jun 15.
Published in final edited form as: Biochem Biophys Res Commun. 2012 May 15;422(4):653–657. doi: 10.1016/j.bbrc.2012.05.046

Figure 1. MDCK cells expressing tandem zyxin LIM plasmids.

Figure 1

(A) Schematic of zyxin and tandem LIM sequences. (B) Western blot of stable MDCK cell lines expressing no exogenous zyxin (WT), the full-length zyxin (Zyx), and single (s), tandem dimer (td), and tandem trimer (tri) LIM sequence. All constructs are tagged with GFP. The membranes were blotted with GFP (top) and tubulin (bottom). (C) Live-cell images of MDCK cells expressing GFP-tagged full-length zyxin, single LIM, tandem dimer LIM, and tandem trimer LIM constructs. Scale bar 10 µm.