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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Bone Miner Res. 2012 Jul;27(7):1585–1597. doi: 10.1002/jbmr.1601

Figure 5. Runx1 expression in periosteum-derived mesenchymal stem cells is necessary for cartilaginous callus formation during fracture healing.

Figure 5

(A) The calluses of Runx1F/+, Prx1-Cre; Runx1F/+, and Prx1-Cre; Runx1F/F mice at day 7 postfracture were stained with Safonin O (Red: cartilage) and Fast Green (Green: mineralized tissue) to identify the cartilaginous (*) and bony (#) callus formation. (B) Histomorphometric assessment was performed to measure the size of the cartilaginous and bony callus. n=4-5 mice per group (C) Gene expression of Runx1, Runx2, Runx3, and Sox9 within the fracture calluses of Runx1F/+ and Prx1-Cre; Runx1F/F was measured by real-time PCR and normalized to β-actin. n=4 mice per group with a duplicate format of real-time PCR. *p<0.05 vs Runx1F/+; #p<0.05 vs Prx1-Cre; Runx1F/+.