Table 2. Principal biological annotation and functional terms identified by gene set enrichment analysis.
The genes differentially expressed in 4 children with AOM due to Spn relative to their pre-infection healthy stage were analyzed. Only of genes with a high fold change (< 0.5 and > 2 fold) in expression and enrichment score >1.3 and P< 0.05 occurring in all 4 children are shown.
| Cluster* 1 Enrichment Score: 2.01 | ||||
|---|---|---|---|---|
| Term** | Count | % | P Value | Fold Enrichment |
| GO:0016064, immunoglobulin mediated immune response | 14 | 0.77 | 0.0019 | 2.62 |
| GO:0019724, B cell mediated immunity | 14 | 0.77 | 0.0027 | 2.52 |
| GO:0002460, adaptive immune response based on somatic recombination of immune receptors | 16 | 0.88 | 0.0078 | 2.10 |
| GO:0002250, adaptive immune response | 16 | 0.88 | 0.0078 | 2.10 |
| GO:0002449, lymphocyte mediated immunity | 15 | 0.82 | 0.0079 | 2.16 |
| GO:0002455, humoral immune response mediated by circulating immunoglobulin | 9 | 0.49 | 0.0090 | 2.93 |
| GO:0006958, complement activation, classical pathway | 8 | 0.44 | 0.0205 | 2.78 |
| GO:0002252, immune effector process | 21 | 1.15 | 0.0409 | 1.58 |
| GO:0002443, leukocyte mediated immunity | 15 | 0.82 | 0.0426 | 1.76 |
| Total | 128 | |||
| Cluster 2 Enrichment Score: 1.671 | ||||
| Term | Count | % | P Value | Fold Enrichment |
| GO:0045935, positive regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolic process | 82 | 4.49 | 0.0064 | 1.33 |
| GO:0051173, positive regulation of nitrogen compound metabolic process | 84 | 4.60 | 0.0070 | 1.32 |
| GO:0045941, positive regulation of transcription | 74 | 4.05 | 0.0099 | 1.32 |
| GO:0010557, positive regulation of macromolecule biosynthetic process | 84 | 4.60 | 0.0102 | 1.30 |
| GO:0031328, positive regulation of cellular biosynthetic process | 87 | 4.76 | 0.0118 | 1.28 |
| GO:0009891, positive regulation of biosynthetic process | 88 | 4.82 | 0.0122 | 1.28 |
| GO:0010628, positive regulation of gene expression | 75 | 4.11 | 0.0136 | 1.30 |
| GO:0045944, positive regulation of transcription from RNA polymerase II promoter | 47 | 2.57 | 0.0635 | 1.28 |
| Total | 621 | |||
| Cluster 3 Enrichment Score: 1.54 | ||||
| Term | Count | % | P Value | Fold Enrichment |
| GO:0000902, cell morphogenesis | 51 | 2.79 | 0.0070 | 1.45 |
| GO:0000904, cell morphogenesis involved in differentiation | 37 | 2.03 | 0.0097 | 1.53 |
| GO:0048667, cell morphogenesis involved in neuron differentiation | 32 | 1.75 | 0.0146 | 1.54 |
| GO:0032989, cellular component morphogenesis | 54 | 2.96 | 0.0147 | 1.37 |
| GO:0048858, cell projection morphogenesis | 36 | 1.97 | 0.0171 | 1.48 |
| GO:0032990, cell part morphogenesis | 36 | 1.97 | 0.0308 | 1.42 |
| GO:0030030, cell projection organization | 48 | 2.63 | 0.0404 | 1.32 |
| GO:0007409, axonogenesis | 28 | 1.53 | 0.0415 | 1.46 |
| Total | 322 | |||
| Cluster 4 Enrichment Score: 1.31 | ||||
| Term | Count | % | P Value | Fold Enrichment |
| GO:0055074, calcium ion homeostasis | 30 | 1.64 | 0.0106 | 1.61 |
| GO:0055066, di-, tri-valent inorganic cation homeostasis | 36 | 1.97 | 0.0120 | 1.52 |
| GO:0006874, cellular calcium ion homeostasis | 29 | 1.59 | 0.0132 | 1.60 |
| GO:0030005, cellular di-, tri-valent inorganic cation homeostasis | 34 | 1.86 | 0.0159 | 1.51 |
| GO:0055065, metal ion homeostasis | 31 | 1.70 | 0.0191 | 1.53 |
| GO:0006875, cellular metal ion homeostasis | 29 | 1.59 | 0.0304 | 1.49 |
| Total | 189 |
*
Number of functional annotation cluster (enrichment score >1.3).
*
Number of functional annotation terms(enrichment 0f >1.3 and P<0.051.These genes were analyzed using DAVID, software [http://david.abcc.ncifcrf.gov].