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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Pain. 2012 May 19;153(7):1514–1524. doi: 10.1016/j.pain.2012.04.015

Figure 2.

Figure 2

NR1 and TRPV1 expression and co-immunoprecipitation in TG. (A) immunohistochemical staining of TG sections showing FB-labeled muscle afferents, TRPV1- and NMDA-labeled neurons as indicated. The arrows indicate muscle afferents that co-express both NR1 and TRPV1. Scale bar: 50 μm. (B) (left) Immunoblot (IB) using anti-TRPV1 or anti-NR1 antibody following immunoprecipitation (IP) of TG extract with anti-NR1 antibody. (Right) Reverse IP with anti-TRPV1 antibody and IB with anti-NR1 or anti-TRPV1 antibody. (C) The same IB-IP protocols were used to show co-immunoprecipitation of TRPV1 and NR2B subunit.