Figure 6.
The involvement of PKC in NMDA-induced serine phosphorylation of TRPV1 and mechanical hyperalgesia. (A) Examples of immunoblots and averaged relative p-Ser/TRPV1 following the NMDA (200 μM) treatment with and without GF109203X (10 μM). (B) Examples of immunoblots and averaged relative p-Ser/TRPV1 following the NMDA treatment with and without DMSO (0.1 %), the vehicle control for GF109203X. The samples were collected at the 15 minute time point (*, p < 0.05 in one-way ANOVA; n = 6-8 per group in A and B). (C) The effects of GF109203X pretreatment on NMDA induced mechanical hyperalgesia (*, p < 0.05 in group effects with respect to vehicle condition in two-way ANOVA; #, p < 0.05 to the baseline in post-hoc test; n = 6-8 per group).