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. Author manuscript; available in PMC: 2012 Jun 19.
Published in final edited form as: Curr Cancer Drug Targets. 2009 Aug 1;9(5):595–607. doi: 10.2174/156800909789056962

Fig. (2). Impact of KP772 on RR activity and cellular dNTP pools.

Fig. (2)

(A) Influence of KP772 on 3H-cytidine incorporation was measured in HL60 cells after 24 h drug incubation. DNA was isolated and radioactivity determined as described under Material and Methods. Values given are means ± standard deviation of one representative experiment out of three performed in triplets. ** Significantly different from the control cells (p < 0.01, by Student’s t-test) (B) HL60 cells were incubated for 24 h with the indicated concentrations of KP772. dNTPs were isolated and measured by HPLC as described under Material an Methods. Values given are means ± standard deviation of 3 determinations out of one representative experiment. Significant dose-concentration response * p < 0.05, ** p < 0.01, p < 0.001 by one-way ANOVA (C) Expression levels of RR subunits were determined by Western blotting in the indicated cell lines after 24 h drug treatment. Antibodies used are described under Material and Methods.