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. Author manuscript; available in PMC: 2012 Jun 19.
Published in final edited form as: Curr Cancer Drug Targets. 2009 Aug 1;9(5):595–607. doi: 10.2174/156800909789056962

Fig. (4). Radical scavenging properties of KP772.

Fig. (4)

(A) After 30 min preincubation with KP772 (0.5 μM and 1 μM) or NAC (2 mM), KB-3-1 cells were treated for 72 h with the indicated concentrations of H2O2. Vitality was determined using MTT assay. Values given are means ± standard deviation of 3 determinations out of 3 experiments. Statistical significance was determined by unpaired Student’s t test (*** p<0.001). (B) Impact of KP772 and NAC on DNA damaging activity of H2O2 was measured by Comet assay as described under Material and Methods. (C) Influence of 30 μM KP772 or 2 mM NAC on the intracellular ROS production in KB-3-1 cells after 1 h incubation with H2O2 (0.1 mM) was determined using the ROS indicator DCF-DA. Fluorescence was measured by flow cytometry. One representative experiment out of three delivering comparable results is shown. Statistical significance in comparison to H2O2 treatment was determined by unpaired Student’s t test; ** p< 0.01, *** p<0.001.