Table 1.
Restriction types (RTs) and eBURST grouping of Italian and Mexican maize-rhizosphere B. cenocepacia IIIB isolates.
| RT | Isolate name a | RFLP type b | IIIB-specific PCR c | Alleles at the following loci | ||||
|---|---|---|---|---|---|---|---|---|
| recA | gyrB | fliC | cepIR | dsbA | ||||
| Group 1 | ||||||||
| 74 | MDII-143p | I | + | 9 | 10 | 2 | 4 | 1 |
| 112 | MDII-151p | I | + | 9 | 8 | 2 | 4 | 1 |
| Group 2 | ||||||||
| 100 | MDIII-B604 | I | + | 9 | 9 | 4 | 4 | 1 |
| 17 | MexII-968 | I | + | 9 | 9 | 1 | 4 | 1 |
| Group 3 (RT-4-complex) | ||||||||
| 115 | MDIII-B752 | J' | + | 10 | 9 | 2 | 7 | 1 |
| 21 | MDIII-P378 | J' | + | 10 | 4 | 2 | 1 | 1 |
| 21 | MexII-864 | J' | + | 10 | 4 | 2 | 1 | 1 |
| 3 | MexII-57 | J' | + | 10 | 10 | 2 | 1 | 2 |
| 7 | MexII-60 | J' | + | 10 | 10 | 2 | 8 | 2 |
| 7 | MexII-857 | J' | + | 10 | 10 | 2 | 8 | 2 |
| 6 | MexII-815 | J' | + | 10 | 10 | 2 | 1 | 1 |
| 53 | MexII-845 | J' | + | 10 | 8 | 1 | 1 | 1 |
| 31 | MexII-940 | J' | + | 10 | 9 | 1 | 1 | 1 |
| 36 | MexII-945 | J' | + | 10 | 4 | 2 | 6 | 1 |
| 4* | MexII-967 | J' | + | 10 | 9 | 2 | 1 | 1 |
| 67 | MexII-974 | J' | + | 10 | 8 | 2 | 6 | 1 |
| 57 | MexII-994 | J' | + | 10 | 8 | 2 | 1 | 2 |
| Group 4 | ||||||||
| 50 | MexII-867 | ukd | + | 11 | 8 | 6 | 1 | 1 |
| 46 | MexII-976 | I | + | 9 | 8 | 6 | 1 | 1 |
| Singletons | ||||||||
| 80 | MCII-168 | J' | + | 10 | 5 | 3 | 6 | 2 |
| 27 | MDII-129r | I | + | 9 | 4 | 2 | 6 | 2 |
| 75 | MDIII-B250 | J' | + | 10 | 8 | 4 | 4 | 1 |
| 114 | MDIII-B716 | I | + | 9 | 4 | 2 | 7 | 1 |
| 28 | MDIII-P410 | I | + | 9 | 4 | 5 | 7 | 2 |
| 22 | MDIII-P461 | J' | + | 10 | 4 | 2 | 7 | 2 |
| 29 | MDIII-T228 | I | + | 9 | 4 | 4 | 1 | 1 |
| 103 | MDIII-T521 | J' | + | 10 | 9 | 4 | 4 | 2 |
| 70 | MexII-206 | uke | + | 13 | 10 | 5 | 7 | 2 |
| 47 | MexII-264 | J' | + | 10 | 2 | 1 | 2 | 2 |
| 48 | MexII-828 | J' | + | 10 | 2 | 2 | 4 | 1 |
| 33 | MexII-863 | J' | + | 10 | 4 | 6 | 10 | 2 |
* The asterisk denotes the ancestral clone of the clonal complex.
a Italian isolates: MCII, MCIII, MVP-C1, MVP-C2, MDII, MDIII-B, MDIII-P, MDIII-T, followed by the number of isolate. Mexican isolates: MexII, followed by the number of isolate.
b recA RFLP with HaeIII.
c The presence and absence of specific PCR amplification for B. cenocepacia IIIB is indicated by a signus "+" and "-", respectively.
d, e uk, unknown RFLP profiles: restriction profiles never recovered among BCC reference strains examined [56].