Figure 2.

MHV Replication Is Not Inhibited by RNase L Signaling

(A–C) BMMs from B6 or RNase L^−/− mice were infected with MHV-A59, SeV, or EMCV (1 PFU/cell). At the indicated time points, RNA was isolated from cell lysates and viral mRNA expression quantified by qRT-PCR (n = 3). Relative viral mRNA expression levels were expressed as 2^−ΔCT, relative to β-actin mRNA [ΔC_T = C_{T(gene of interest)} – C_T(β-actin)].

(D) Cells infected with MHV-A59 were lysed by freeze-thawing and titers determined by plaque assay (n = 3).

See also Table S1.