Figure 3.

The MHV ns2 Mutant Replicates to the Same Extent as WT Virus in BMM from RNase L^−/− but Not PKR^−/− mice

(A and B) BMMs, derived from RNase L^−/− or PKR^−/− mice were infected (0.01 PFU/cell). At the indicated times, titers of viruses in the cell lysates combined with supernatants were determined by plaque assay (n = 3).

(C) BMMs derived from B6 or RNase L^−/− mice were infected (MOI = 0.01 PFU/cell). At 48 hr p.i., the cells were stained with anti-MHV N antibody and Hoechst. The scale bar represents 100 μm.

See also Figure S1.