Figure 6.

MHV ns2 Enhances Virus Replication In Vivo by Inhibiting RNase L Signaling

(A) Four-week-old B6 or RNase L^−/− mice were inoculated intrahepatically with A59 or ns2-H126R (200 PFU/mouse). At 3, 5, and 7 d.p.i., organs were harvested and homogenized, and virus titers were determined. The dashed line represents the limit of detection and the error bars represent the SEM (n = 5). Asterisks indicate that differences are statistically significant (^∗p < 0.05 and ^∗∗∗p < 0.001).

(B) Liver sections from mice sacrificed at 5 d.p.i. were stained with anti-MHV N monoclonal antibody. The scale bar represents 200 μm. Sections are representative of two sections from each of three animals from each group.

See also Figure S3.