Figure 4.

Temporal variability of signalling events in sister cells reveals stochasticity. NIH3T3 cell clone stably expressing IRF-7–CFP or IRF-7–TagRFP together with IFN-β–tGFP were infected with 80 HAU/ml NDV for 1 h (A–D, n=38 sister-cell pairs) or transfected with poly I:C (5 μg/ml) (E–H, n=36 sister-cell pairs) and subjected to time-lapse microscopy (20 min interval). Sister-pair analysis was carried out for IRF-7 nuclear translocation and IFN-β expression onset. Coloured dots indicate the frequency of data points. (A, E) Time of IRF-7 nuclear translocation onset (T_sig) in sister-cell pairs. (B, F) Time intervals between IRF-7 nuclear translocation and IFN-β–tGFP expression onset (T_gen) among sister cells. (C, G) Time elapsed from cell division to IRF-7 nuclear translocation (T_sig−T_div) of sister cells. (D, H) Time elapsed from cell division to IFN-β–tGFP expression (T_sig+T_gen−T_div). Source data is available for this figure in the Supplementary Information.

Source data for Figure 4ABCD ^{(1.3KB, txt)}

Source data for Figure 4EFGH ^{(1.6KB, txt)}