Skip to main content
. 2012 May 15;52(10):2082–2090. doi: 10.1016/j.freeradbiomed.2012.03.022

Fig. 2.

Fig. 2

Rapid activation of eNOS by ascorbate is linked to changes in eNOS phosphorylation. (A) EA.hy926 cells and (B) HUVECs were treated with 100 μM ascorbate for 5 min to 1 h. Western blot and subsequent densitometric analyses were performed to detect and quantify (phospho-) eNOS protein levels. One representative blot is shown. Band intensities are normalized to tubulin and expressed as fold untreated control (⁎p<0.05; ⁎⁎p<0.01; ⁎⁎⁎p<0.001; ns, not significant; mean±SEM, n=5 for (A) and n=3 for (B)). (C) EA.hy926 cells were treated for 1 h with the indicated concentrations of ascorbate and subjected to Western blot analysis for the detection of (phospho-) eNOS levels. One representative blot is shown. Band intensities are normalized to actin and expressed as fold untreated control (⁎p<0.05; ⁎⁎p<0.01; ⁎⁎⁎p<0.001; ns, not significant; mean±SEM, n=4).