Figure 2.

Circulating ChE activities in wt and mdx mice. ChE activities in the sera of adult male wt (C57Bl/10J) and mdx □ mice were assayed by: (A,B) Measurement using the Ellman colorimetric method, with ATCh as substrate and the selective inhibitors, iso-OMPA, for measurement of AChE (striped columns), or BW284c51, for measurement of BChE (plain columns) (A); BChE activity was also measured using BTCh (B); (C,D) The radiometric method with ³H-ACh as substrate and with iso-OMPA for AChE (C), or with BW284c51 for BChE (D). (E) CK levels in the sera of wt and mdx mice. Activity values represent the mean ± SEM, of samples from 6 to 8 mice per group for ChEs and of 12 animals per group for CK. Activities in mdx sera differed from wt significantly: The BChE level in mdx sera was reduced compared to wt (^*p < 0.01 in B and < 0.05 in D), while AChE in mdx sera was elevated compared with wt (^*p < 0.05 in A,C). As expected, CK levels in mdx sera were elevated, compared to those in the wt sera (E, ^*p < 0.001).