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. 2012 Jun 19;5:73. doi: 10.3389/fnmol.2012.00073

Figure 3.

Figure 3

Effect of orchidectomy and T-replacement appears to be on BChE, not on AChE. Activities in wt sera of BChE (A,B) and AChE (C) were determined colorimetrically (A) or radiometrically (B,C) with appropriate substrates and selective inhibitors as described in the legend to Figure 2. Values are the mean ± SEM for 6–8 samples per group (sham group, n = 3). BChE levels in gonadectomized animals (dashed line in A) differ significantly from those in intact, T-treated and sham animals: Data were analyzed by One-Way ANOVA, showing statistical significance in A (p « 0.0002) and in B (p « 0.0001), with Tukey's post-hoc revealing significant differences (*) between castrated male (cas, shaded column) and the other three groups: intact male (male) (p < 0.0003), castrated with T-replacement (cas + T) (p < 0.0002) or sham (p < 0.0002). All other comparisons showed insignificance (p > 0.05): male compared to cas + T (p = 0.086), or to sham (p = 0.07), and cas + T compared to sham (p = 0.92). Similarly, post-hoc test in B showed significant difference between cas and the other groups: male (p < 0.001), cas + T (p < 0.0004), and sham (p < 0.001), which were very similar. No significant changes were observed in AChE activity (C).