Fig. 1.

Reversible inhibition of transient receptor potential melastatin 7 (TRPM7) channels by 303 μM Mg²⁺ in an inside-out patch excised from a Jurkat T lymphocyte. Mg²⁺ was applied multiple times directly to the inner face of the membrane. Current reduction was fully reversible after each application. Note that with third and fourth applications the current magnitude is gradually reduced due to slow rundown. Membrane was held at −90 mV. Representative experiment chosen from n = 7 patches. [Mg²⁺]_free, free Mg²⁺ concentration.