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. Author manuscript; available in PMC: 2012 Jun 19.
Published in final edited form as: Glia. 2010 Jul;58(9):1082–1093. doi: 10.1002/glia.20989

Figure 5.

Figure 5

OSM-induced release of TNF-α from microglia is neurotoxic. Primary microglia were incubated with media alone (UN) or with OSM (10 ng/ml) for 24 h. Four hundred μl of supernatant from each condition was added to primary cortical neuron cultures and incubated for 24 h at 37°C. Where indicated, supernatants were incubated with antibodies against TNF-α or total mouse IgG for 2 h at 37°C before being added to neurons. After 24 h, neuronal viability was assessed using the MTT Reagent. Absorbance values of each sample were measured in duplicate at 570 nm. Cell viability readings of neurons incubated with supernatants from media alone-treated microglia were set to 100%. Results show the average % cell viability for each condition +/− S.D. from 2 independent experiments. * p-values < 0.05 were considered statistically significant.