Fig. 1.
Steady-state properties of S742E and S747E mutant CLH-3b channels expressed with functional or kinase dead (KD) GCK-3. GCK-3-mediated phosphorylation of S742 and S747 hyperpolarizes CLH-3b activation voltage, slows hyperpolarization-induced current activation, and decreases whole cell current amplitude at hyperpolarized voltages (11). A: steady-state current amplitude and current-to-voltage relationships (left) and representative whole cell current traces (right). B: activation voltages. C: voltage-dependent activation kinetics measured as 50% rise time of S742E and S747E mutant channels expressed with or without functional GCK-3. Steady-state channel properties of the two mutants are similar. I, current; Vm, membrane potential. Values are means ± SE (n = 6–7).