Fig. 2.

Swelling-induced changes in the activity of wild-type (WT) CLH-3b channels and S742E and S747E mutants coexpressed with functional GCK-3. Cells were swollen at time 0 by exposure to a 250 mosM bath solution. Rates of swelling-induced increase of current amplitude (A), depolarization of activation voltage (B), and decrease of 50% rise time (C) were significantly (P < 0.05) slower in S747E mutant channels compared with either wild-type channels or the S742E mutant. Values are means ± SE (n = 4–7).