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Effect of expression of wild-type, S742E, or S747E linkers containing the F669A mutation on functional properties of wild-type CLH-3b. Cells were cotransfected with 1.5 μg, 0.075 μg, and 4 μg of wild-type CLH-3b, GCK-3, and linker cDNAs, respectively. A: whole cell current amplitude and current-to-voltage relationships. B: activation voltages. C: 50% rise time. D: rates of and shrinkage-induced inactivation. Values are means ± SE (n = 7–8).
