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Western blot detection of the transient receptor potential vanilloid 4 (TRPV4) channel in porcine lens epithelium (epi). Rat kidney and pig kidney were used as positive controls. Either 200 μg of lens epithelium or 100 μg of kidney homogenate protein was loaded to each lane. When the primary antibody was blocked with the specific antigen peptide (right), it failed to recognize the TRPV4 band in the lens epithelium.
