Fig. 2.

The influence of TRPV4 antagonists RN 1734 and HC 067047 on the ATP release response to hyposmotic Krebs solution (200 mosM). Intact lenses were exposed to hyposmotic Krebs solution for 10 min in the presence or absence of the TRPV4 antagonist added 20 min beforehand: RN 1734 (10 μM) (A); HC 067047 (0.1–100 μM) (B). Using the data in B, an IC₅₀ of 2.5 μM was calculated for inhibition of hyposmotic solution-induced ATP release by HC 067047. ATP in the bathing solution was measured by luciferin-luciferase assay. Values are means ± SE of results from 6–8 lenses. ***P < 0.001, significant difference from control; ^###P < 0.001, significant difference from hyposmotic treatment.