Fig. 9.

The influence of TRPV4 inhibitor RN 1734 on the Src family kinase (SFK) phosphorylation response to hyposmotic Krebs solution (200 mosM). Intact lenses were exposed to hyposmotic solution for 2 min in the presence or absence of RN 1734 (10 μM) added 20 min beforehand, after which the epithelium was removed, homogenized, and subjected to Western blot analysis for SFK phosphorylation at Y416. β-Actin was probed as a loading control. A: representative Western blot. Con, control; Hypo, hyposmotic solution. B: relative phospho-SFK (pY416 SFK) band density (compared with control). Values are means ± SE of results from 3 independent experiments. ***P < 0.001, significant difference from control.