Fig. 6.

Induction of cyclin D expression during acinar cell culture. Acinar cells were cultured for the time indicated in control media (solid line) or with 1 nM CCK (dashed gray line). RNA or protein was extracted and samples were analyzed by quantitative real-time PCR or Western blot. The expression of cyclin D1, D2, and D3 mRNA (A–C) and protein (D–F) was measured. Results are expressed as fold increase compared with the fresh acini and means ± SE of 4–6 individual experiments. For D–F, duplicate culture wells were run on gels and imaged, and after quantification the image was spliced to show only 1 lane per condition as described in materials and methods. For this reason a white line is placed between the lanes. **P < 0.01; *P < 0.05 compared with the value of fresh acini. ‡‡P < 0.01; ‡P < 0.05 compared with the non-CCK-treated group at the same time point (dashed vs. solid line).