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Top: gene expression of RAS elements upstream ANG I metabolism, angiotensinogen (AGT) and cathepsin D, in hGEnCs verified by Western blotting. Bottom: expression of the RAS enzyme aspartyl aminopeptidase (DAP; which was identified by tandem MS) in hGEnCs verified by Western blotting. Each lane was loaded with 25 μg of cell protein, except hKidney lane of blot probed for cathepsin D (5 μg).
