Fig. 4.

A: MALDI-TOF mass spectrum generated from conditioned media collected after incubation of human glomerular endothelial cells with 1 μM ANG I in the absence (top) or presence (bottom) of 100 μM captopril [ANG-converting enzyme (ACE) inhibitor] for 4 h. Inset: quantitative inspection of ANG II [1,046 mass-to-charge ratio (m/z)] peaks using 100 nM AQUA-ANG II (1,052 m/z) as internal standard. B: quantification of observed differences at 4 h (average from 5 separate batches of cells). C: disappearance curve of ANG I, with or without ACE inhibition. Values are means ± SE. *P < 0.0001 vs. control. ¥P < 0.01 vs. control.