Fig. 5.

A: MALDI-TOF mass spectrum generated from conditioned media collected after incubation of hGEnCs with 1 μM ANG I in the absence (top) or presence (bottom) of 100 nM Z-prolyl prolinal [prolylendopeptidase (PEP) inhibitor] for 4 h. Inset: quantitative inspection of ANG 1–7 (899 m/z) peaks using 100 nM AQUA-ANG 1–7 (905 m/z) as internal standard. B: quantification of observed differences at 4 h (average from 3 separate batches of cells), including the effect of 10 μM SCH39370 [neprilysin (NEP)] inhibitor. C: disappearance curve of ANG I, with or without PEP inhibition. Values are means ± SE. *P < 0.01 vs. control.