Fig. 9.

A: MALDI-TOF mass spectrum generated from conditioned media collected after incubation of hGEnCs with 1 μM ANG III in the absence (top) or presence (bottom) of 1 mM bestatin [aminopeptidase N (APN) inhibitor] for 4 h. Inset: quantitative inspection of ANG IV (775 m/z) peaks using 50 nM AQUA-ANG IV (781 m/z) as internal standard. B: quantification of observed differences at 4 h (average from 3 separate batches of cells). C: disappearance curve of ANG III, with or without APN inhibition. Values are means ± SE. *P < 0.001. ¥P < 0.01 vs. control.