Fig. 8.

AQP2 and Na-K-2Cl cotransporter 2 (NKCC2) abundance in rat outer medullary (OM) tissue of the Ctrl group and Dox-treated group. Non-pair-fed as well as pair-fed animals were evaluated. A: representative immunoblots showing abundance of AQP2 and NKCC2 in rat OM tissue lysates. The right arrow points to the non-gly AQP2 band. The right brace signifies gly AQP2 bands. An equal amount of total protein from a different rat tissue sample was loaded into each lane. B: densitometric analysis of Western blots from 3 non-pair-fed and 2 pair-fed cohorts of animals for AQP2 (left) and NKCC2 (right). Data were normalized for the average densitometry of untreated animals in each group and adjusted for alkaline phosphatase as a control protein. Separate densitometric analysis was performed for gly and non-gly AQP2. Data are expressed as means ± SE (non-pair-fed n = 18 treated, 12 controls; pair-fed n = 10; *P < 0.05).