Western blot showing PTEN expression in a panel of human tumour lines. PTEN mutant lines are shown in red and PTEN wild type lines in black. PTEN genotypes are as follows: DU145 (prostate), PTEN wild type; MCF7 (breast), PTEN wild type; PC3 (prostate) p.R55fs*1 homozygous; MDAMB468 (breast), p.A72fs*5 homozygous; A172 (glioma), p.R55fs*1 homozygous; UM-UC3 (bladder), p.M1*404del homozygous; RPMI-7951 (melanoma) p.null, c.1-79del79 homozygous; HCC70 (breast), p.F90fs*9 homozygous (see
Table S1 of Supporting Information for complete genotypes).