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. 2012 Mar 27;302(12):E1550–E1559. doi: 10.1152/ajpendo.00462.2011

Fig. 3.

Fig. 3.

Effects of PU.1 knockdown on transcription levels of inflammatory cytokines and NADPH oxidase. A: 3T3-L1 cells expressing shPU.1 or shScr were differentiated in the presence of rosiglitazone. The expression of differentiation markers (PPARγ, C/EBPα, and GLUT4) in cells was detected by Western blot analyses. B: transcription levels of inflammatory cytokines were detected using real-time RT-PCR method. Data are means ± SE (n = 6). C: mRNA levels of components of NADPH oxidase were measured using real-time RT-PCR method. Data are means ± SE (n = 6). D: NADPH oxidase activity in cell homogenates was measured. Data are means ± SE (n = 6). *P < 0.05; **P < 0.01. RLU, relative luminescence unit.