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Regulation of glucose uptake in PU.1 knockdown adipocytes. A: both basal and 100 nM insulin-stimulated 2-deoxy-d-[1-3H]glucose uptake in 3T3-L1 adipocytes expressing shPU.1 or shScr were determined. Data are means ± SE (n = 9). B: the protein levels of GLUT1 in these cells were detected by Western blot analysis. *P < 0.05; **P < 0.01.
