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PU.1 regulates adipocyte lipolysis. 3T3-L1 adipocytes expressing shPU.1 or shScr were serum starved for 4 h and then treated with insulin (100 nM) for 2 h, followed by addition of isoproterenol (10 μM). One hour later, the supernatant was harvested and free glycerol content determined. Data are means ± SE (n = 6). *P < 0.05; **P < 0.01 between control and PU.1 knockdown cells; #P < 0.01 between samples with and without insulin treatment.
