Fig. 2.

Steatohepatitis is attenuated in CCR2^−/− mice. WT and CCR2^−/− mice were fed the CSAA diet (CS, n = 5) or the CDAA diet (CD, n = 10) for 22 wk. A: H-E, Oil Red O, α-SMA, and Sirius red staining are shown. Steatosis, inflammatory cell infiltration, and fibrosis were attenuated in CCR2^−/− mice fed the CDAA diet. Original magnification, ×200 for H-E and Oil Red O staining, ×400 for α-SMA and Sirius red staining. Bar = 100 μm. B: nonalcoholic fatty liver disease (NAFLD) activity score. C: hepatic triglyceride (TG) and total cholesterol (TC) and free fatty acid (FFA) content. D: serum alanine transferase (ALT) levels. E: hepatic mRNA expression of inflammatory cytokines. F: Sirius red-positive area was measured on 10 low-power (x100) fields/slide and quantified with the use of NIH imaging software. G: hepatic mRNA expression of fibrogenic genes was measured by quantitative real-time PCR. Genes were normalized to 18S RNA as an internal control. TIMP, tissue inhibitor of metalloproteinase; PAI, plasminogen activator inhibitor. H: insulin resistance was assessed by a homeostasis model assessment of insulin resistance (HOMA-IR). Data represent means ± SD, *P < 0.05.