Fig. 5.

Q-RT-PCR analysis of oxidant-associated genes in kidney following recovery from AKI. Total RNA derived from whole kidney of sham-operated rats and rats following recovery from I/R using “oxidant stress and anti-oxidant” pathway arrays in kidneys of sham and post-AKI rats (see methods). A: data are expressed as Log(2)-normalized ratios relative to the expression in sham; therefore, greater expression in AKI vs. sham corresponds to a positive value and a lower expression in AKI is represented by a negative value. Shown are data from 14 of the 84 genes evaluated (the 7 most upregulated and 7 downregulated). A 95% confidence interval of Log(2) ratios was determined to be ±1.6 and was used to define significance (dotted line). B, C, and D: data from individual Q-PCR verification of array results for the GPX3, dual-oxidase 1 (Duox1), and lactoperoxidase (LPO) genes, respectively, in a wider number of samples (n = 6 per group) and in APO-treated rats. Data are expressed as means ± SE of 2^ΔCt values normalized to the RPLP1 genes. *P < 0.05 relative to sham control by Student's t-test.