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Interstitial collagen 4 deposition is increased in the stenotic kidney of WT, compared with Smad3 KO mice. Collagen 4 immunostaining of renal cortex from WT (A–C) and Smad3 KO (D–F) mice 6 wk following surgery is shown. A and D: sham control kidneys. B and E: RAS contralateral kidneys. C and F: RAS stenotic kidneys. Graph values are means ± SE. *P < 0.05 vs. WT Sh. †P < 0.05 vs. KO Sh. ‡P < 0.05 vs. WT St.
