Fig. 4.

Papillary LRC from H2B-GFP mice migrate and proliferate in response to SDF-1. A: LRC isolated from H2B-GFP mice and grown in serum-free media formed spheres. B: 7 days after the addition of SDF-1, many cells moved a considerable distance from the spheres (arrow), which, in turn, diminished in size or disappeared altogether (C). D: in contrast, in the absence of SDF-1 (control), very few cells (arrows) separated from the spheres and stayed close to them. Bars = 100 μm. E: distance at which cells located from the edge of the spheres in the absence (control) or presence of SDF-1 in the media after 7 days (*P < 0.01; 3 spheres were analyzed in each individual experiment with n = 7 for control and n = 8 for SDF-1 cultures). F: when grown in culture media containing 0.5% FCS, cell number increased markedly in response to bFGF and modestly but significantly in response to SDF-1 (*P < 0.01; **P < 0.001).