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The rate constant R1/[E] (s−1) as a function of temperature for the interconversion of CO2 and bicarbonate catalyzed by variants of HCA II. Wild type = black filled square; TS1 = red filled rhombus; TS2 = dark blue triangle; TS3 = brown square; TS4 = light blue filled square and TS5 = green filled triangle. Data have been separated on the ordinate to avoid superposition of points. Solutions contained 10 mM of all species of CO2 and 100 mM HEPES at pH 7.6.
