Fig. 3.
Actin alignment in endothelial monolayers on microposts depends on flow conditions. A–C: representative immunofluorescence images of 160 × 160-μm HPAEC monolayers on microposts after exposure to static (A), laminar (B), or disturbed flow conditions (C) for 14 h (green: actin, gray: microposts). D–F: rose plots of actin alignment for static (D), laminar (E), and disturbed flow conditions (F). Actin alignment in the direction of flow was evident for laminar flow conditions. Plots in D–F are from 15 samples/condition and from 3 replicate experiments. Scale bar = 40 μm.