Fig. 1.

Blood pressure response and endothelial nitric oxide synthase (eNOS) expression in DOCA-salt hypertensive mice. A: blood pressure of sham-operated control or DOCA-salt hypertensive mice was measured using a tail-cuff method. B: upregulation of eNOS protein expression in DOCA-salt hypertensive mice. Proteins were extracted from sham-operated control of DOCA-salt hypertensive aortas using a Tris-based homogenization buffer containing 1% Triton and protease inhibitors. Cellular proteins (20 μg) were separated with SDS-PAGE. eNOS protein expression was determined using a specific monoclonal antibody raised against human eNOS (BD Transduction Laboratory). Top: representative Western blot. Bottom: grouped densitometric data from 6 separate experiments. **P < 0.01 vs. sham.