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. 2012 Apr 4;302(11):R1313–R1326. doi: 10.1152/ajpregu.00086.2012

Fig. 2.

Fig. 2.

Effects of Arc injections of Lep-SAP and B-SAP on feeding and body weight changes in response to leptin and on Arc α-MSH and suprachiasmatic nuclei (SCN) AVP immunohistochemistry. A: food intake, expressed as a percentage of intake on the baseline day (1 day prior to the start of leptin or aCSF injections). B: body weight on days 1, 2, and 3 (as a percentage of body weight on the baseline day) of daily lateral ventricular leptin (2.5 μg in 3.0 μl) or artificial cerebrospinal fluid (aCSF; 3 μl) injections (n = 6–8 rats per group). #P < 0.01; *P < 0.001 vs. B-SAP rats. C: coronal sections through basal hypothalamus. Top row shows α-MSH-immunoreactivity in the Arc (2.8 to 3.0 mm caudal to bregma) of a rat injected with B-SAP (left) or Lep-SAP (right). Bottom row shows arginine vasopressin (AVP)-immunoreactivity in the SCN (0.6 to 0.7 mm caudal to bregma) of rats injected into the Arc with B-SAP (left) or Lep-SAP (right). Calibration bar = 20 μm, or 40 μm for AVP, α-MSH, respectively.