Table 1.
Analysis of feeding rhythms
| Late Dynamic | Static |
||||
|---|---|---|---|---|---|
| Phase | LD (4 days) | LD 1 (3 days) | DD (28 days) | LD 2 (7 days) | LL (16 days) |
| Rhythms | |||||
| Period, h | |||||
| B-SAP | 24.4 ± 0.03 | 24.8 ± 0.3 | 25.5 ± 0.3 | ||
| Lep-SAP | 22.4 ± 0.9$ | 24.1 ± 0.2 $ | ND | ||
| Amplitude (Robustness) | |||||
| B-SAP | 45.2 ± 7.0 | 20.0 ± 2.6 | 10.5 ± 0.5 | ||
| Lep-SAP | 16.0 ± 3.0 $ | 26.0 ± 7.0 $ | ND | ||
| % of Rhythmic rats | |||||
| B-SAP | 100 | 100 | 67 | ||
| Lep-SAP | 29 | 43 | 0 | ||
| Food Intake | |||||
| 24-h, g | |||||
| B-SAP | 26.1 ± 0.9 | 25.0 ± 2.2 | 26.2 ± 0.8 | 21.0 ± 1.3# | 20.6 ± 3.1# |
| Lep-SAP | 36.1 ± 5.9*# | 31.6 ± 1.5* | 37.4 ± 2.8*# | 26.3 ± 1.9*# | 23.5 ± 3.6# |
| 12-h in Light, g | |||||
| B-SAP | 4.8 ± 1.0 | 4.4 ± 0.9 | 6.0 ± 0.7 | 5.3 ± 1.0 | 6.9 ± 1.4# |
| Lep-SAP | 12.4 ± 3.3* | 10.5 ± 1.3* | 16.1 ± 1.8*# | 6.7 ± 1.0# | 8.4 ± 1.4 |
| 12-h in Dark, g | |||||
| B-SAP | 21.3 ± 0.9 | 20.6 ± 1.5 | 20.2 ± 0.5 | 15.7 ± 0.9# | 13.7 ± 1.8# |
| Lep-SAP | 23.7 ± 2.7 | 21.1 ± 0.9 | 21.3 ± 1.3 | 19.6 ± 1.0* | 15.1 ± 2.4# |
| 12-h in Light/24-h, % | |||||
| B-SAP | 18.4 ± 3.4 | 16.7 ± 2.7 | 22.6 ± 2.1# | 25.1 ± 4.4# | 32.4 ± 3.5# |
| Lep-SAP | 31.4 ± 4.7* | 32.8 ± 3.1* | 42.3 ± 2.6*# | 25.2 ± 3.1# | 36.3 ± 2.5 |
Feeding rhythm analyses and food intake during the late dynamic phase (4 days) and the four stages of the static phase: 3 days of 12:12-h light-dark (LD1) cycle, 28 days of continuous dark (DD), 7 days of second LD (LD2), and then 16 days of continuous light (LL). For feeding rhythm analysis, only the individual rats rhythmic by Lomb-Scargle periodogram were used to calculate the averages of period and amplitude (robustness). Because of the free run during DD and LL, only the first 2 days of food intakes of those stages are presented in order to evaluate the transitions between stages for subjective light or subjective dark. Note that for LD2, the period is not 24 h because rats were transitioning from DD during the 7 days.
Significant difference using a t-test (P < 0.05) between paired data for B-SAP and Lep-SAP rats.
Significant difference (P < 0.05) between LD1 values at the beginning of the static phase and another value in a different lighting condition of the same group. ND, no individual rat was found to be rhythmic. $Sample size for Lep-SAP rats was too small for statistical comparison.