Figure 3. (A and B) ALP marker expression pattern in transfected H4 cells showed a co-staining of LAMP-1, LAMP-2A and, LC3 and α-synuclein positive aggregates in the SynT-aggregation model (A; indicated by arrows, representative α-synuclein aggregates are magnified), whereas no changes of ALP marker expression pattern were observed in WT-Syn control transfected cells compared with nontransfected neighboring cells (B). (C) In temporal cortex tissue of α-synuclein transgenic animals LAMP-2A co-labeled mainly the outer rim of α-synuclein positive inclusions (arrows), whereas co-labeling of LC3 and α-synuclein could be detected either at the rim (arrows), or homogenously distributed. LAMP-1 was not found in α-synuclein inclusions in vivo (data not shown). Scale bars: 10 µm.